Z Microsystems GLIDER 20 Uživatelský manuál

LeicaMacroFluo™User manual

Leica MacroFluo – Assembly1010 447 041 (500mm)11 507 80810 445 36310 445 63110 445 36710 446 35910 447 185 (500 mm)10 447 05710 446 30110 445 38710 44

11Leica MacroFluo – Assembly2.2 Assembly sequenceAssemble the components in the following order: – Stand: Base, column with focusing drive, focus-ing

12Leica MacroFluo – AssemblyFig. 2 Leica MacroFluo™ with zoom 6:11 Base of stand (transmitted-light base HL RC™shown)2 Focusing drive with column (sho

13Leica MacroFluo – Assembly2.6 Zoom systemThe MacroFluo™ can be combined with a 6:1 (Z6 APO) or 16:1 (Z16 APO) zoom system.Zoom system ➜ Carrier for

14Fig. 4 Z6 APO on the left: Mount the objective onthe diaphragm ring of the zoom system.Z16 APO on the right: Objective mounted on thefine focusing (

15Leica MacroFluo – AssemblyFig. 5 Hinged arm with UV protection screen1 Hinged arm 2 Clamp with clamping screw for fastening tocarrier3 Central rotar

16Leica MacroFluo – Assembly2.10 Instrumentation of nosepiece discThe nosepiece disc can be fitted with a total of4 filter blocks (filter system). At

The 4 positions of the nosepiece disc are eachlabeled with the numbers 1 through 4 on the leftside of the dovetail guide. The numbers can beseen in th

18Leica MacroFluo – Assembly2.11 Connection of digital or video cameras The trinocular tube is observation and photo tubein one and features a photo m

19왘 Activate the lamp housing for the incident lightfluorescence illuminator at the supply unit andadjust the burner.3.1.3 Disconnecting from the powe

2Leica MacroFluo – User manualDear User,Thank you for your faith in our product. We hope your work with it is bothenjoyable and successful. Leica Macr

20Fig. 11 Trinocular tube HC L3TP 1 Push rod with 3 switching positions for deflect-ing the light to the eyepieces or to the con-nected camera2 Eyebas

213.4 Iris diaphragmThe integrated iris diaphragm is used for continu-ous adjustment of the depth of field withoutchanging the magnification. Reducing

223.7 Adjusting diopter settings (parfocality)With adjustable eye lenses at the eyepieces, theobserver can compensate his or her defectivevision. The

23Fig. 13 Parfocality 1 Adjustable tubes for adjusting the eyebase 2 Eye lenses for adjusting the diopter settings 3 Adjustable eyecups4 Working dista

243.8 Incident light fluorescence illuminatorThe patented Leica zero-pixel-shift technologyensures an exact convergence for the superposi-tion of diff

25Leica MacroFluo – Operation3.8.5 Filter blocksThe filter turret (14.1) can accommodate up to fourfilter blocks (ch. 2.10). These filter blocks conta

26Leica FluoCombi III™ – Appendix4.1 Filter blocks for macro-fluorescence4. AppendixFilter block Fluorochrome Excitation Excitation filter Dichromatic

27Leica MacroFluo – Appendix4.2 Assignment of fluorochromes and filter blocksFluorochromes Filter block– Acridine-blue A– Acridine-yellow I 3, H 3–

28Leica MacroFluo – Appendix– Calcein-blue A– Calcium crimson Y 3– Calcium green K 3, I 3, L 5– Calcium orange M 2, N 2.1– Calcofluor white H 3,

Leica MacroFluo – Appendix– FITC (FlouresceinIsoThioCyanate) I 3, H 3, K 3, L 5– FITC/Ethidiumbromide I 3, L 5, N 2.1– FITC (selective) L 5– Texas

3Leica MacroFluo – OverviewsOverview of the instrumentFig. 1 Leica MacroFluo™ with zoom system 16:11 Base of stand (transmitted-light base HL RC™shown

30Leica MacroFluo – Appendix– Phycoerythrin (PE) N 2.1, N 3– Primulin O D– Procion Yellow D, E 4, H 3– Propidiumiodide N 2.1– Pyronin B N 2.1, M

31Leica MacroFluo – Appendix4.3 Optical dataVisual dataEyepieces HC Plan 10×/25 (11507808)Tube factor: 1.25× trinocular tube FSA HC L 3TPIris diaphrag

32Designation Leica MacroFluo™ with 6.3 : 1 zoom Leica MacroFluo™ with 16 : 1 zoomMicroscope type Macroscope with vertical beam path, apochromatic zoo

33Leica MacroFluo – AppendixOptical accessoriesObjectives Type/magnification factor Working distance Max. field of view øPlanapo 1× 97mm 35mmPlanapo 0

34Leica MacroFluo – Appendix4.5 DimensionsDimensions in mm

35Leica MacroFluo – Appendix8.3°50.6°∅6337.2118.5∅58∅58∅9.537.2∅585.3°44.6°∅63155.6∅58∅9.5Leica Z6 APOLeica Z16 APO

36Leica MacroFluo – Appendix∅4538.2612M42x3∅58∅4512M42x3∅58954.8∅4541.5612M42x3∅58∅4532612M42x3∅58∅4525617M42∅54∅386.5M50(Fine Focus Interface)(Achrom

Leica Microsystems – the brandfor outstanding productsLeica Microsystems – an international company with a strong network of customer servicesAustrali

4Page1 Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51.1 The user manual . . . . . . . . . . . . . . . . . . . . . . . . .

5Leica MacroFluo – User manual1. Safety concept1.1 The user manualIncluded with your Leica MacroFluo™ is an inter-active CD-ROM with all pertinent use

Leica MacroFluo – General notes61.1.2 Symbols usedWarning of a dangerThis symbol indicates especially important infor-mation that, if not observed,– c

7Permitted usesThe Leica MacroFluo™ allows for examining,manipulating, sorting and documenting entireunprepared fluorescent objects. The high resolu-t

8Leica MacroFluo – AssemblyRepairs and servicing– Only Leica Microsystems-trained service tech-nicians, or technical specialists assigned by theperson

– when assembling or disassembling the lamphousing,– before opening the lamp housing,– when changing the high-pressure mercuryburner and remaining par